#=GF ID UvrD-helicase #=GF AC PF00580.22 #=GF DE UvrD/REP helicase N-terminal domain #=GF AU Bateman A;0000-0002-6982-4660 #=GF SE MRC-LMB Genome group. #=GF GA 23.00 23.00; #=GF TC 23.00 23.00; #=GF NC 22.90 22.90; #=GF BM hmmbuild HMM.ann SEED.ann #=GF SM hmmsearch -Z 47079205 -E 1000 --cpu 4 HMM pfamseq #=GF TP Domain #=GF RC Structure of Swiss:P09980 #=GF RN [1] #=GF RM 9288744 #=GF RT Major domain swiveling revealed by the crystal structures of #=GF RT complexes of E. coli Rep helicase

Bacterial UvrD helicase. It is involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair. It unwinds DNA duplexes with 3'-5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present.

It is involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair. It unwinds DNA duplexes with 3'-5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is present. UvrD helicase-RNA polymerase interactions are governed by UvrD's carboxy-terminal Tudor domain. Coronavirus: Find the latest articles and preprints Sign in or create an account Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis.It is active on a wide range of DNA substrates and, along with its thermostability (active to 70°C), Tte UvrD Helicase has been demonstrated to be a useful additive for improving Tte-UvrD Helicase. 50 rxns ( 20 µg/ml ) - Unavailable in your region: M1202S. Tte-UvrD Helicase. 50 rxns-1 + Unavailable in your region .).

UvrD helicase activity can be activated through interactions with the MutL protein (34, 35) that is required for methyl-directed mismatch repair (36). We have shown that a single MutL dimer is sufficient to activate the UvrD monomer helicase and increase its processivity as well as stimulate the UvrD dimer helicase (37). The most known SF1A helicases are Rep and UvrD in gram-negative bacteria and PcrA helicase from gram-positive bacteria. The most known Helicases in the SF1B group are RecD and Dda helicases. [24] They have a RecA-like-fold core. #=GF ID UvrD-helicase #=GF AC PF00580.22 #=GF DE UvrD/REP helicase N-terminal domain #=GF AU Bateman A;0000-0002-6982-4660 #=GF SE MRC-LMB Genome group.

The MutL protein is a homodimeric DNA-stimulated ATPase that plays a central role in MMR in Escherichia coli. UvrD is a helicase that is widely conserved in gram-negative bacteria. A uvrD homologue was identified in Mycobacterium tuberculosis on the basis of the homology of its encoded protein with Escherichia coli UvrD, with which it shares 39% amino acid identity, distributed throughout the protein.

2008-03-15 · The well studied E. coli UvrD helicase (helicase II) unwinds DNA in the 3' to 5' direction. Unlike many other helicases, the UvrD helicase is capable of melting fully duplex molecules (DNA fragment with blunt ends) as well as nicked circular DNA molecules.

Previously, we have developed an isothermal DNA amplification method using the UvrD helicase from E coli (1). … Helicase-dependent amplification (HDA) is an isothermal in vitro DNA amplification method based upon the coordinated actions of helicases to separate double-stranded DNA and DNA polymerases to synthesize DNA. Previously, a mesophilic form of HDA (mHDA) utilizing the Escherichia coli UvrD helicase, DNA polymerase I Klenow fragment, two accessory proteins, MutL and single-stranded DNA … 2012-03-09 UvrD helicase, but not Rep helicase, efficiently disrupts RecA–ssDNA nucleoprotein filament.

The Rep helicase is needed during bacteriophages M13 and ΦX174 replication (Takahashi et al, 1979), the UvrD helicase ensures the replication of Gram‐negative rolling‐circle plasmids (Bruand and Ehrlich, 2000) and the PcrA helicase ensures the replication of Gram‐positive rolling‐circle plasmids (Petit et al, 1998; Anand et al, 2004).

Although a UvrD monomer can translocate along single-stranded DNA, a UvrD dimer is needed for processive helicase activity in vitro. E. coli MutL, … Bacterial UvrD helicase. It is involved in the post-incision events of nucleotide excision repair and methyl-directed mismatch repair. It unwinds DNA duplexes with 3'-5' polarity with respect to the bound strand and initiates unwinding most effectively when a single-stranded region is … 2019-08-13 2017-11-14 Veaute, X. et al. UvrD helicase, unlike Rep helicase, dismantles RecA nucleoprotein filaments in Escherichia coli.

The helicase ac-tivity of the Tte-UvrD is described, as are the effects of the Tte-MutL protein on unwinding reactions catalyzed by Tte-UvrD helicase.
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It is a 1441 amino acid long protein with a conserved UvrD domain from 36 – 801 amino UVRD_HELICASE_CTER, PS51217; UvrD-like DNA helicase C-terminal domain profile (MATRIX) Sequences in UniProtKB/Swiss-Prot known to belong to this class: 257. detected by PS51217: 255 (true positives) undetected by PS51217: 2 (2 false negatives and 0 'partial') Other sequence(s) in UniProtKB/Swiss-Prot detected by PS51217: NONE. 2019-01-14 UvrD helicase-RNA polymerase interactions are governed by UvrD's carboxy-terminal Tudor domain.

2018-04-17 UvrD helicase from P. falciparum and the Rep helicase is required for bacteriophages M13 and ΦX174 replication (Takahashi et al., 1979). The role of Rep The P. falciparum genome is available from the Plasmodium helicase has been elucidated in MMR. The progression of repli Direct imaging of single UvrD helicase dynamics on long single-stranded DNA. Nature Communications, 2013. Hamza Balci. Taekjip Ha. Kyung Lee. Haifeng Jia. Timothy Lohman.
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Escherichia coli UvrD is a superfamily 1 helicase/translocase involved in multiple DNA metabolic processes including methyl-directed mismatch DNA repair. Although a UvrD monomer can translocate along single-stranded DNA, a UvrD dimer is needed for processive helicase activity in vitro. E. coli MutL, …

Sequence archive. Help. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. 2018-04-17 UvrD helicase from P. falciparum and the Rep helicase is required for bacteriophages M13 and ΦX174 replication (Takahashi et al., 1979). The role of Rep The P. falciparum genome is available from the Plasmodium helicase has been elucidated in MMR. The progression of repli Direct imaging of single UvrD helicase dynamics on long single-stranded DNA. Nature Communications, 2013. Hamza Balci. Taekjip Ha. Kyung Lee. Haifeng Jia. Timothy Lohman.